產品描敘

​RNA Experiment, For RNA Isolation

產品說明

C2H6N4S
M.W.: 118.16
99.0% Min.
Loss on Drying: <0.3% Melting Range: 118-121℃ Heavy Metals (as Pb): <0.0005% Solubility: Clear and Colorless Exceptionally fine preparation Chaotropic agent and strong denaturant; solubilizes cells. Guanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published. Chirgwin, J.M. et al. (1979) Biochemistry 18, 5294-5299227 Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227 Isolation of RNA using guanidinium salts. Isolation of non-degraded RNA from RNase-rich tissues. 包裝規格

1. 250G
2. 250G*4